Journal: Cell Chemical Biology

Article Title: Directed evolution of potent neutralizing nanobodies against SARS-CoV-2 using CDR-swapping mutagenesis

doi: 10.1016/j.chembiol.2021.05.019

Figure Lengend Snippet:

Article Snippet: Post antigen binding, the cells were washed once with ice-cold PBSB and incubated with mouse anti-Myc (1000x dilution) and chicken anti-His (1000x dilution; Invitrogen, PA1-9531) antibodies on ice for 20 min. Post primary incubation, the cells were washed with ice-cold PBSB and incubated with goat anti-mouse IgG AF488 (200x dilution) and donkey anti-chicken IgY F(ab’) 2 fragment AF647 (500x dilution; Jackson ImmunoResearch, 703-606-155) antibodies on ice for 4 min. Post-secondary incubation, the cells were washed once with ice-cold PBSB and evaluated by flow cytometry.

Techniques: Virus, Recombinant, Luciferase, Protease Inhibitor, DC Protein Assay, Plasmid Preparation, Software, Magnetic Beads, Gel Extraction, Purification